Develop and Distribute Reference Materials
CPPSI develops and distributes reference materials that facilitate the identification of plant pathogen strains. Reference materials are comprised of pathogen strain or race differentiating host sets, reference pathogen strains and an informative white paper. The white paper explains what the disease is, the nature of resistance to the disease, how to work with strains or races of the pathogen causal agent, how to evaluate resistance and susceptibility to a pathogen strain or race in a seedling test, and how to identify the pathogen strain or race in question. In addition, the white paper lists contacts for ordering seeds and reference pathogen strains or races.
Differential hosts are sets of commercial plant varieties, each containing one or more genes for resistance to strains or races of a given plant pathogen. By inoculating an unknown strain of a given plant pathogen across the differentiating hosts, the strain can be identified based on the pattern of susceptible or resistant responses to that strain. Reference strains are known characterized isolates of a given pathogen strain. The response of the differential hosts to the reference strain or strains is compared to that of your strain in question to verify identification. Reference strain responses are also used to substantiate that the test results are correct.
Library of Reference Materials
To launch the initiative, CPPSI members deployed a library of reference materials for four disease systems: tomato tobacco mosaic virus, pepper bacterial spot, melon fusarium wilt, spinach downy mildew, lettuce downy mildew, pepper Tomato spotted wilt virus and tomato Tomato spotted wilt virus This was done to show that the CPPSI system of distribution would work for each type of plant pathogen: a virus, bacterium, fungus and an obligate plant pathogen. Today, we are expanding that library.
Seed of the differential hosts for each of these hosts can be ordered online through the USDA Germplasm Resources Information Network (GRIN). Refer to the GRIN links noted on the CPPSI website and in each of the white papers. For some hybrid host differentials, a Material Transfer Agreement (MTA) may be required to order seed. The GRIN order form indicates which differential hosts might require an MTA and the name of the person to be contacted for arranging an MTA.
To acquire the reference plant pathogen strains and races please refer to the contact information provided on the CPPSI website and in each white paper. It is the responsibility of those requesting pathogen strains to ensure they have the necessary permits to receive the plant pathogen strains and races by contacting the USDA Animal and Plant Health Inspection Service.